IgG1 Fc (AA 98-324) Protéine
Aperçu rapide pour IgG1 Fc (AA 98-324) Protéine (ABIN7275250)
Antigène
Type de proteíne
Origine
Source
Pureté
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Attributs du protein
- AA 98-324
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Fonction
- Mouse IgG1 Fc Protein
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Séquence
- Val98-Lys324
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Attributs du produit
- Recombinant Mouse IgG1 Fc Protein is expressed from HEK293 without tag.It contains Val98-Lys324.
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Stérilité
- 0.22 μm filtered
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niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- The affinity constant of 15.09 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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IgG1 Fc (AA 104-330) protein
Origine: Humain Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgG1 Fc protein (Fc Tag)Origine: Souris Hôte: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
IgG1 Fc proteinOrigine: Humain Hôte: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. WB
IgG1 Fc (AA 100-330) protein (DYKDDDDK Tag)Origine: Humain Hôte: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Stock
- -20 °C,-80 °C
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Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Date de péremption
- 12 months
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- IgG1 Fc
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Sujet
- Recombinant murine Fc fragment contains the hinge region, CH2, and CH3 region of the mouse IgG1 isotype. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.
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Poids moléculaire
- 25.7 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
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UniProt
- P01868
Antigène
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